(2/4) The above left shows the frozen state of the sample consisting of numerous single ice crystals. Among them, we need to secure one single ice crystal to evaluate its melting (Tm)- and freezing (Tf)-temperatures, as the TH is the difference between them [1, 2, 3]. Please watch the movie 2 showing such securing process for 10 mg/mL insect AFP solution [3]. When the crystal count becomes fewer by fast warming (e.g. 30 degC/min), you should successively apply slight warming and occasional cooling (0.1~5 degC/min) so as to remain only one single ice crystal. Note that Tm is the temperature where the single ice crystal shrinks to be melted, while Tf is the temperature where the crystal starts "bursting growth" [1, 4, 5]. (->next) (->home) |
|
